The Determination of Ammonia and Amide Nitrogen in Tobacco by the Use of Permutit.*

The accurate determination of the preexisting ammonia or of the amide nitrogt.n of tobacco is rendered difficult by the volatility of nicotine which distils over more or less completely when the standard procedures for the estimation of these forms of nitrogen are employed. A number of attempts has been made to devise methods whereby corrections for the nicotine content of such distillates may be calculated but none of these is entirely satisfactory.